Abstract

岎攝晄慡惈巕偺偆嬠Fusarium oxysporum偼丄搚忞揱愼惈偺怉暔昦尨嬠偱丄怉暔崻晹偐傜怤擖偟丄摫娗撪傪枲墑偟丄慡恎傪堔挏丒屚巰偝偣傞丅杮嬠偼丄巁慺暘埑偑掅偄偲憐掕偝傟傞怉暔偺摫娗撪偱惗堢偡傞偙偲偐傜丄摫娗撪偱偼寵婥屇媧傪峴偆偙偲偑悇嶡偝傟傞丅傑偨丄杮嬠偼寵婥屇媧偺侾偮偱偁傞傾儖僐乕儖敪峺擻傪帩偮偙偲傕抦傜傟偰偄傞丅偦偙偱丄杮尋媶偱偼傾儖僐乕儖敪峺偲昦尨惈偲偺娭楢惈偵偮偄偰挷嵏偡傞偨傔丄岲婥屇媧偲傾儖僐乕儖敪峺偺暘婒揰晅嬤偱婡擻偡傞僺儖價儞巁僨僇儖儃僉僔儔乕僛乮PDC乯偵拝栚偟偨丅F. oxysporum偱偼PDC堚揱巕偼枹摨掕偱偁偭偨偨傔丄嬤墢庬偱偁傞F. graminearum偺僨乕僞儀乕僗忋偺丄懠嬠僺儖價儞巁僨僇儖儃僉僔儔乕僛偲憡摨惈偺崅偄婡擻枹抦僞儞僷僋幙乮#XM_390010.1乯傪僐乕僪偡傞堚揱巕忣曬傪婎偵丄弅廳PCR丄TAIL-PCR偵傛偭偰丄F. oxysporum偐傜3554bp偺抐曅傪僋儘乕僯儞僌偟偨丅偙偺DNA抐曅拞偵偼丄3偮偺僄僋僜儞偐傜側傞悇掕ORF乮1710 bp乯偑懚嵼偟丄PDC偲憐掕偝傟傞570傾儈僲巁偐傜側傞僞儞僷僋幙傪僐乕僪偟偰偄傞偲峫偊傜傟偨丅偙偺堚揱巕傪pdc1偲偟偨丅PDC1偼丄傾儈僲巁攝楍偱F. graminearum偺悇掕僞儞僷僋幙偲96.1%丄Asperugillus fumigatus偺PDC乮#XM_74419.1乯偲63.5亾丄A. oryzae偺PDC乮#AF098293.1乯偲62亾丄Saccharomyces cerevisiae偺PDC1乮#NC_001144.4乯偲47%偺憡摨惈傪帵偟偨丅傑偨丄PDC偵崅偔曐懚偝傟傞傾儈僲巁儌僠乕僼乮-GDG-偐傜-NN-乯偑懚嵼偟偨乮aa. 448-476乯丅僎僲儈僢僋僒僓儞夝愅偺寢壥丄pdc1偼F. oxysporum僎僲儉忋偵1僐僺乕懚嵼偟偨丅