僜儔儅儊壩傇偔傟昦嬠Olpidium viciae偺18S rDNA晹暘墫婎攝楍偺曄惈嵻擹搙岡攝僎儖揹婥塲摦乮DGGE乯朄傪梡偄偨夝愅
Acquisition of 18S rDNA partial sequence in Olpidium viciae Kusano, the blister disease pathogen of broad bean utilizing denaturing gradient gel electrophoresis (DGGE)
堫尒孿屽丒嶳壓廋堦*丒帥壀丂揙丒桳峕丂椡
Keigo Inami丆Shuichi Yamashita, Tohru Teraoka丆Tsutomu Arie

Abstract

僣儃僇價栧偵懏偡傞Olpidium viciae偼丄Kusano(1912)偵傛偭偰尒弌偝傟偨愨懳婑惗惈偺搚忞昦尨嬠偱丄僜儔儅儊傗僄儞僪僂側偳偺儅儊壢怉暔偵摿堎揑偵婑惗偟丄壩傇偔傟昦傪堷偒婲偙偡丅杮朚偱偼朳憤敿搰偱偺敪惗偑曬崘偝傟偰偄傞偑丄嬤擭偼偦偺敪惗偑尭彮偟偰偄傞丅偦偙偱丄O. viciae偺宯摑妛揑埵抲傪暘巕惗暔妛揑偵夝愅偡傞偙偲傪寁夋偟偨丅偦偺偨傔偵丄2008擭5寧丄朳憤敿搰偱壩傇偔傟昦偵滊昦偟偨僜儔儅儊慻怐傪嵦廤丄偙偺姶愼梩慻怐偐傜僩乕僞儖DNA傪拪弌偟偨丅偙偺DNA傪拻宆偵18S rDNA偺晹暘抐曅傪PCR偵傛偭偰憹暆偟偨丅偟偐偟丄梡偄偨僾儔僀儅乕僙僢僩乮FF390, 5乫-CGATAACGAACGAGACCT-3乫亄 FR1-GC, 5乫-CCCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGCCGAICCATTCAATCGGTAIT-3乫乯偱偼丄僜儔儅儊偺摨椞堟傕憹暆偡傞偨傔丄O. viciae偺抐曅偲嬫暿偱偒側偄丅寬慡僜儔儅儊梩偲滊昦僜儔儅儊梩桼棃偺PCR憹暆抐曅傪曄惈嵻擹搙岡攝僎儖揹婥塲摦乮DGGE乯偱僶儞僪僷僞乕儞傪斾妑偟丄O. viciae姶愼梩偐傜偺傒専弌偝傟傞抐曅傪僔乕僋僄儞僔儞僌偟偨丅摼傜傟偨墫婎攝楍偲摨懏偺O. brassicae傗丄懠懏偺僣儃僇價偱偁傞Synchytrium spp.側偳傪暪偣偰暘巕宯摑夝愅傪峴偭偨偲偙傠丄O. viciae姶愼梩偐傜偺傒専弌偝傟偨抐曅偺攝楍偑O. brassicae偺傕偺偲椶帡偟偰偍傝丄摼傜傟偨墫婎攝楍偑O. viciae偺18S rDNA偺晹暘攝楍偱偁傞偙偲偑帵嵈偝傟偨丅

*搶嫗戝妛戝妛堾

戞俉夞巺忬嬠暘巕惗暔妛僐儞僼傽儗儞僗乮俀侽侽俉擭侾侾寧侾俉擔丄嬥戲巗乯億僗僞乕敪昞