寀岝僞儞僷僋幙儅乕僇乕傪棙梡偟偨旝惗暔杊彍帒嵽Talaromyces sp. KNB-422偲僀僱偽偐昪昦嬠偺憡屳嶌梡偺娤嶡
Observation of interactions between Talaromyces sp. KNB422-GFP, a biocontrol agent and Gibberella fujikuroi, the causal pathogen of 乪Bakanae 乫 disease using fluorescent proteins
壛摗椇岹丒嶰戭懽巌*丒寴愇廏柧*丒帥壀丂揙丒桳峕丂椡
Akihiro Kato, Taiji Miyake*, Hideaki Tateishi*,Tohru Teraoka, Tsutomu Arie

梫巪

亂栚揑亃
旝惗暔帒嵽偵傛傞昦奞杊彍偺嶌梡婡嶌偵偼丄峈嬠暔幙偺惗嶻丄怘嬠丒梟嬠嶌梡丄嫞崌丄怉暔偺昦奞偵懳偡傞掞峈惈偺桿摫側偳偑曬崘偝傟偰偄傞丅桳岠側棙梡朄偺妋棫傗岠壥偺埨掕壔丄埨慡惈妋曐偺娤揰偐傜丄旝惗暔帒嵽偺嶌梡婡嶌傪柧傜偐偵偟偰偍偔偙偲偼廳梫偱偁傞丅
條乆側惗暔偱儅乕僇乕偲偟偰棙梡偝傟偰偄傞嫮壔椢怓寀岝僞儞僷僋幙乮EGFP乯偵壛偊偰丄愒怓寀岝僞儞僷僋幙乮DsRed2乯傪巺忬嬠偱敪尰偝偣傞僾儔僗儈僪儀僋僞乕pAK2-HYG傪嶌弌偟丄寀岝傪儅乕僇乕偵偟偨嬠懱偺娤嶡宯傪妋棫偟偨乮壛摗丄2008乯丅偙傟偵婎偯偒丄杮尋媶偱偼堎側傞寀岝僞儞僷僋幙敪尰姅傪梡偄偰旝惗暔帒嵽偲昦尨嬠偺憡屳嶌梡傪壜帇壔偟丄旝惗暔帒嵽偺嶌梡婡嶌偺夝柧偵岦偗偨in planta偱偺娤嶡傪峴偭偨丅
亂曽朄亃
巺忬嬠梡EGFP敪尰儀僋僞乕pMK412乮Watanabe丄2007乯偍傛傃pAK2-HYG乮壛摗丄2008乯傪梡偄偰丄旝惗暔杊彍帒嵽Talaromyces sp. KNB-422偺EGFP敪尰姅乮KNB-422-GFP乯偲僀僱偽偐昪昦嬠偺DsRed2敪尰姅乮G. fujikuroi-RFP乯傪嶌弌偟偨丅僗儔僀僪僇儖僠儍乕偱椉嬠偺憡屳嶌梡傪娤嶡偟偨丅偝傜偵G. fujikuroi-RFP墭愼僀僱乮昳庬丗抁嬧朧庡乯庬巕傪KNB-422-GFP偱張棟乮1.0亊106 cfu/ml暘惗巕寽戺塼傊偺怹捫乯偟丄堢昪抜奒偵偍偗傞椉嬠偺憡屳嶌梡傪娤嶡偟偨丅
亂寢壥亃
丂懳洺攟梴偟偨僗儔僀僪僇儖僠儍乕偱偼丄KNB-422-GFP偑G. fujikuroi-RFP偵岦偗偰嬠巺傪暘婒偝偣偨傝嬠巺傪姫偒偮偐偣偨傝丄愊嬌揑偵嶌梡偟偰偄傞條巕偑娤嶡偝傟偨丅椉嬠偺岎嵎丄愙怗晹埵偱偼G. fujikuroi-RFP偺嵶朎幙偺寀岝偑徚柵偟偰偄傞尰徾偑尒傜傟偨丅堢昪抜奒偺僀僱庬巕忋偱偼丄KNB-422-GFP偑栢妅昞柺忋偱嬠巺傪怢挿偟丄摿偵僀僱偺敪夎晹埵廃曈偵懡偔偵嬠巺偑懚嵼偟丄偦偺堦晹偺嬠巺偼廤崌偟偰嬠巺夠傪宍惉偟偰偄偨丅偙偺嬠巺夠偺拞偱G. fujikuroi-RFP偺嬠巺偵KNB-422-GFP偺嬠巺偑棈傑偭偰偄傞條巕偑娤嶡偝傟偨丅KNB-422-GFP偺張棟偵傛偭偰丄僀僱梩忊傊偺G. fujikuroi-RFP偺怢揥偑尭彮偟丄偽偐昪昦偵傛傞搆挿摍偺昦挜傕梷惂偝傟偰偄傞偙偲偐傜丄KNB-422偑庬巕栢妅昞柺忋偱G. fujikuroi傪怘嬠偟丄忋晹傊偺怢揥傪杊偖偙偲偑KNB-422偺嶌梡婡嶌偱偁傞偲敾抐偟偨丅

乮嫟摨尋媶撪梕乯

*僋儗僴丂Kureha
擔杮擾栻妛夛戞俁係夞婰擮戝夛乮俀侽侽俋擭俁寧侾俋擔丄暥嫗嬫乯岥摢敪昞